The Cell sorting and Flow Cytometry Shared Resource Lab provides solid experimental knowledge with high performance technology for analytical multicolor flow cytometry and cell sorting to all research groups within our Institute.
- Sustained operator expertise with the BD FACSAria III cell sorter ensures optimal instrument setup and maintenance and offers experiment-tailored, aseptic bulk or single-cell sorting. The sorter is configurated with 3 lasers (488, 633, 405nm) and enables detection of up to 12 parameters. Several cell types with multiple markers at different expression levels can be sorted at high purity and well-preserved viability for downstream experiments. The various efforts inside the Resource Lab include sorting mainly of immune cell subsets like neutrophils, monocytes, macrophages, dendritic cells, NK-, T- and B-cells next to hematopoietic progenitor cells, platelets, cells derived from tissue/organ homogenates, transduced cell lines and cell nuclei or apoptotic microvesicles.
- Our institute also offers an access to BD FACSCanto II and BD LSRFortessa X-20 cell analyzers that deliver high performance and multicolor analysis. The BD FACSCanto II is configurated with 3 lasers (488, 633, 405nm) and enables simultaneous detection of up to 8 parameters. The BD LSRFortessa X-20 is configured with 5 lasers (355, 405, 488, 561, 640nm) and enables the detection of up to 20 parameters simultaneously. Thus, BD Fortessa X-20 is a powerful instrument that allows our researchers to detect different hematopoietic cell types and their activation status in tissues.
